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空间分辩生物电化学发光及成象技术研究

批准号20275025 学科分类土壤化学 ( D070103 )
项目负责人周顺桂 负责人职称 依托单位苏州大学
资助金额0.00
万元
项目类别青年科学基金项目 研究期限2003 年 01 月 01 日 至
2005 年 12 月 01 日
中文主题词电化学发光;中性介质;增敏;淬灭,生物ECL传感
英文主题词Electrochemiluminescence; Neutral medium; Sensitization; Quench; Bio-ECL-sensing

摘要

中文摘要 研究适合生物反应的中性电化学发光体系及对有关生物反应体系的响应,结合生物化学反应的分子识别、催化等功能,将生物电化学发光检测体系固定至电极的和光纤表面,构成生物电化学发光传感器。在传感器微型化的基础上实现空间分辨检测并经计量学解析获得图象。可望用于生物反应过程跟踪、非均相生物体液、细胞内乃至单个生物分子的检测与表征。
英文摘要 We have taken the characteristic research according to schedule, that is, to gain obviously chemical blowup based on the sensitized ECL of luminol by free-radical process. This work has overcome the obstacle of the strong base medium, and realized the ECL of luminol in the weak acid and neutral medium. Those substances can sensitize the ECL of luminol in the weak acid and neutral medium such as inorganic substances(i.e. oxygenic matters, halogens), organic substances (i.e. cysteine), biological molecules(i.e. enzyme), medium(i.e. microemulsions) and nano-materials(i.e. nano Au, nano Pt and Carbon-nano-tube), and obtain biological ECL by coupling with catalysis oxidation. A lot of biological molecules (i.e. vitamins, flavones, glutathione, hormones and enzymes), which have remarkable quenching effect on the sensitized ECL, has been mentioned, and realized the high sensitive ECL detection. The sensitivity can reach femto-molar level, that has exceeded the anticipate target. We have studied the ECL behavior of luminol on sorts of electrode materials (such as Pt, Au, Carbon, ITO etc.), and have found that ITO is an excellent electrode material. We have modified ECL agent and sensitizing system based on the chemical modification technology, then perfect ECL electrodes are obtained and they can be used in the development of ECL detector. And what's more, some materials, such as cysteine, nano Au(Pt) and carbon-nano-tube, can not only retain sensitizing capability, but also absorb luminol further more, so it is helpful to fabricate the sensitive ECL electrode. The methods of electrodeposite, electropolymerization and physical matrix embedding all can be used in the fabrication, and we have preliminarily developed the sensitive electrode with the biological ECL capability. We also made the digital devices for use in study of ECL and are about to accomplish the work of system integration and software organization.
结题摘要 研究工作富有特色,基于对鲁米诺ECL自由基过程的增敏以获得显著的化学放大作用,克服了局限于强碱性介质的限制,实现了在弱酸性和中性介质中鲁米诺的ECL,无机物(氧系分子、卤素)、有机物(半胱氨酸)、生物分子(酶)、溶液介质(微乳液)、纳米材料(纳米金(铂)、碳纳米管)等均增敏弱酸性-中性介质中鲁米诺的ECL,可以耦合酶催化氧化获得生物ECL。生物分子(如维生素类、黄酮类、谷胱甘肽、激素、酶等)对此增敏的ECL有显著的淬灭作用,实现了灵敏的ECL检测,灵敏度最高达到飞摩尔数量级,超过项目预期目标。研究了鲁米诺在各种电极材料(铂、金、碳、ITO等)上的ECL性能,发现ITO是十分优异的电极材料,并利用化学修饰技术修饰电化学发光试剂及增敏体系,获得效果理想的电化学发光电极,可用于ECL检测器的制作,尤为重要的是,如半胱氨酸、纳米金(铂)、碳纳米管等在电极表面既保持增敏性能,又进一步吸附鲁米诺,极有利于传感电极的制作,电聚合、电沉积、包埋等措施均适用于传感电极的研制,初步研制出具有生物电化学发光性能的传感电极。采用模块方式研制了数字化的电化学发光分析设备部件,即将完成系统集成和软件编制与统调。

成果

序号 标题 类型 作者
1 电化学发光淬灭法测定蜂胶中黄酮总效价 期刊 徐杨|储海红|齐莹莹|狄俊伟|屠一锋|
2 电化学发光淬灭法测定脑白金胶囊中的褪黑素 期刊 储海虹|许海燕|吴莹|胡延维|屠一锋|
3 电化学发光淬灭法测定SOD 期刊 储海虹|吴莹|狄俊伟|屠一锋|
4 Electrogenerated chemiluminescence of CdS spherical assemblies 期刊 Ting Ren|Jin-Zhong Xu|Yi-Feng Tu|Shu Xu|Jun-Jie Zhu|
5 电化学发光淬灭法测定谷胱甘肽 期刊 齐莹莹|吴莹|狄俊伟|屠一锋|

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