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不同空间辐射品质诱发的水稻生物学效应及其对基因组稳定影响的机制研究

批准号31770918 学科分类空间生物学 ( C050304 )
项目负责人孙野青 负责人职称教授 依托单位大连海事大学
资助金额60.00
万元
项目类别面上项目 研究期限2018 年 01 月 01 日 至
2021 年 12 月 31 日
中文主题词空间辐射生物学效应;粒子辐射;基因组不稳定性;空间辐射品质;数据挖掘
英文主题词Space radiation biological effect;Particle radiation;Genomic instability;Space radiation quality;Data mining

摘要

中文摘要 空间辐射损伤预警目前仍未获得根本性进展,这主要源于空间辐射环境的复杂性以及微重力等因素的存在。由于地基实验无法完全模拟真实的空间辐射环境,这也制约了相关研究的进展。伴随高通量检测和大数据分析技术的发展,应用系统论的方法挖掘空间辐射诱因和生物学效应机制可能成为解决上述问题的一个重要突破口。本研究集成具有定位功能的辐射探测片和水稻种子组成的生物堆叠经历了“实践十号”返回式卫星12.5天的飞行。卫星返回后,获取空间辐射品质和水稻从宏观到微观等不同层次上的特征数据。项目拟利用数据挖掘和关联等技术,研究空间辐射在多尺度下的生物学效应,包括表型变化(株高、根长等)、细胞学损伤(染色体畸变等)、以及“组学”变化(功能基因组学、蛋白质组及甲基化测序等)等,揭示空间辐射品质诱发的生物学效应特征,挖掘空间辐射敏感的分子标志物并研究其调控功能,探索不同空间辐射品质诱发內源性ROS的特征及其对基因组不稳定的影响。
英文摘要 At present, space radiation damage prediction has not made significant progress, which is mainly due to the complexity of space radiation environment and the existence of microgravity and other factors. In addition, the ground experiment can not fully simulate the real space radiation environment, which also restricts the progress of related research. With the development of high throughput detection and big-data analysis technologies, it may be an important breakthrough to solve the above problem by using the method of system theory to analyze the space radiation factors and the corresponding mechanisms of biological effects. This project integrated the radiation detectors with positioning function and seeds of rice (Oryza sativa japonica) into the biostacks, which were put into the SJ-10 recoverable satellite. After 12.5 days flight, we can obtain the spatial radiation quality and the characteristic data of rice from macro to micro. The project intends to use the data association and mining and other analytical techniques to study the biological effects of space radiation on rice at multiple scales, including phenotypic changes (such as plant height and root length, etc.), cytological damage (e. g., chromosomal aberration, etc.), and “omic” variation (such as functional genomics, proteomics and methylation sequencings, etc.) to reveal the characteristics of biological effects induced by different space radiation qualities, to mine the molecular markers of radiation sensitivity in space and study their regulatory functions, and to explore the characteristics of endogenous ROS induced by different space radiation qualities and their effects on genomic instability.
结题摘要

成果

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